Research on individuals with dark personalities in the work context has received much attention in the past ten years. However, one challenge remains: creating valid measures that focus on and capture the personality profile of those individuals with possible psychopathic features. The B-Scan 360 Long Form (LF) is a measure of psychopathy created for the workplace and used by others to describe a co-worker, an employee, or a supervisor. Two studies were conducted using online samples to assess the factor structure and validity of the instrument. Results indicated that B-Scan 360 LF facets were internally consistent and unidimensional. Confirmatory factor analyses supported a reliable fifteen facets and four-factor model consistent with the Psychopathic Checklist-Revised four-factor model of psychopathy. The B-Scan 360 LF showed similar patterns of association with FFM traits as other psychopathy measures. The B-Scan 360 LF correlated negatively with supervisor Agreeableness, Conscientiousness, and Emotional Stability. Correlations between the B-Scan 360 LF and Openness were positive. However, correlation between B-Scan 360 LF and Extraversion was non-significant. Although more research is needed to establish the validity of the B-Scan 360 LF, we believe these results show that it is a promising option to measure psychopathy in the workplace.