Past studies have suggested important links between valence and perception and action in physical space. The present study aimed to test the associations between emotional valance for words or faces and manual responses in left- and right-handers. We employed a divided output (bimanual) reaction time paradigm in which participants had to discriminate emotional valance of words or faces presented on the center of the screen. The results revealed that right-handers were faster when responding to positive words or faces with their right hand and to negative words or faces with the left hand, whereas left-handers showed the opposite pattern. These results suggest that the association between space and valence depends on handedness, providing further evidence for the body-specificity hypothesis.