We examined the factor structure, reliability, convergent and criterion validity of the Greek version of the Difficulties in Emotion Regulation Scale (DERS) using data from a community lifespan sample. Exploratory and confirmatory factor analyses revealed a clear six-factor structure consistent with the factor structure evidenced in English-speaking samples. The results also demonstrated adequate classical psychometric properties in terms of internal consistency and test-retest reliability. The scale branches demonstrated convergent and concurrent validity with personality, emotion intelligence abilities and coping strategies. The importance of using culturally validated dimensional measures of emotion regulation is discussed.