In a bimanual-bisequential version of the serial reaction time (SRT) task participants performed two uncorrelated key-press sequences simultaneously, one with fingers of the left hand and the other with fingers of the right hand. Participants responded to location-based imperative stimuli. When two such stimuli appeared in each trial, the results suggest independent learning of the two sequences and the occurrence of intermanual transfer. Following extended practice in Experiment 2, transfer of acquired sequence knowledge was not complete. Also in Experiment 2, when only one stimulus appeared in each trial specifying the responses for both hands so that there was no basis for separate stimulus–stimulus or separate response–effect learning, independent sequence learning was again evident, but there was no intermanual transfer at all. These findings suggest the existence of two mechanisms of sequence learning, one hand-related stimulus-based and the other motor-based, with only the former allowing for intermanual transfer.